N oni (Morinda citrifolia) is a plant that is widely available inIndonesia. It was reported that Morinda citrifolia extracts from seeds, leaves, andfruits have antibacterial properties. Morinda citrifolia leaf extract is said to havean inhibitory effect on the growth of Streptococcus mutans, so further research isneeded on which extraction method is better. Objectives : To determine thedifferences in growth inhibition of Morinda citrifolia leaves ethanol extract withmaceration and soxhlet methods against Streptococus mutans ATCC 25175.Methods : Laboratory experimental research in the form of antibacterial activitytest on Streptococcus mutans ATCC 25175 using diffusion method on blood agar.Bacteria were treated with Morinda citrifolia ethanol extract by maceration andsoxhlet method in a concentration of 100%. Data analysis was performed with oneway analysis of variance (ANOVA). Results : S. mutans ATCC 25175 had asignificant difference (p <0.05) to ethanol extract of Morinda citrifolia leavesmethod by the soxhlet method with a p value of 0.002. Ethanol extract of Morindacitirifolia leaves with maceration method has a higher inhibition zone meancompared to Morinda citrifolia leaves ethanol extract with maceration methodagainst Morinda citrifolia leaves ethanol extract with soxhlet method. The meandifference is 4.87. Conclusion : There is a significance difference between theethanol extract of Morinda citrifolia leaves with maceration and soxhlet methodson the growth inhibition of Streptococcus mutans ATCC 25175.