Pengaruh ekstrak kulit manggis (garcinia mangostana l.) terhadap densitas biofilm treponema denticola atcc 35405 secara in vitro (Laporan Penelitian)

DETAIL KOLEKSI

Pengaruh ekstrak kulit manggis (garcinia mangostana l.) terhadap densitas biofilm treponema denticola atcc 35405 secara in vitro (Laporan Penelitian)

Oleh : Christopher Jeremy

Info Katalog

Nomor Panggil : 617.632 JER p

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2020

Pembimbing 1 : Dr. drg. Trijani Suwandi, Sp. Perio.

Subyek : Periodontics;Periodontal disease - Prevention

Kata Kunci : periodontal disease, mangosteen peel (Garcinia mangostana L.), treponema denticola.

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.
1.	2020_TA_KG_040001600023_Halaman-Judul.pdf
2.	2020_TA_KG_040001600023_Lembar-Pengesahan.pdf
3.	2020_TA_KG_040001600023_Bab-1_Pendahuluan.pdf	4
4.	2020_TA_KG_040001600023_Bab-2_Tinjauan-Pustaka.pdf
5.	2020_TA_KG_040001600023_Bab-3_Kerangka-Teori,-Kerangka-Konsep,-dan-Hipotesis.pdf
6.	2020_TA_KG_040001600023_Bab-4_Metode-Penelitian.pdf
7.	2020_TA_KG_040001600023_Bab-5_Hasil-Penelitian.pdf
8.	2020_TA_KG_040001600023_Bab-6_Pembahasan.pdf
9.	2020_TA_KG_040001600023_Bab-7_Kesimpulan-dan-Saran.pdf
10.	2020_TA_KG_040001600023_Daftar-Pustaka.pdf	4
11.	2020_TA_KG_040001600023_Lampiran.pdf

Abstrak
Abstract

P enyakit periodontal merupakan penyakit gigi dan mulut yang paling banyakditemukan di Indonesia selain karies. Etiologi utama penyakit periodontal adalahplak. Salah satu bakteri dalam plak yang berperan penting dalam patogenesispenyakit periodontal yaitu Treponema denticola. Manggis (Garcinia mangostanaL.) terbukti memiliki sifat antibakteri, antioksidan, dan antiinflamasi. Penelitian inibertujuan untuk mengetahui pengaruh dari ekstrak kulit manggis (Garciniamangostana L.) terhadap densitas biofilm Treponema denticola ATCC 35405.Manggis diperoleh dari Laboratorium Balittro di Bogor, Jawa Barat. Penelitian inidilaksanakan pada Laboratorium Micore FKG Usakti. Bakteri Treponemadenticola dibiakan dalam 96 well-plate selama 1x24 jam kemudian ditetesi ekstrakkulit manggis dengan konsentrasi 100%, 50%, 25%, 12.5%, 6.25%, medium BrainHeart Infusion (BHI) broth (kontrol negatif) dan klorheksidin 0,2% (kontrolpositif). Well-plate tersebut diinkubasi selama 1 jam, 3 jam, dan 24 jam pada suhu37Â° kemudian diberi pewarnaan crystal violet serta dibaca menggunakanmicroplate reader. Uji normalitas dengan metode Shapiro-Wilk menunjukkan dataterdistribusi normal (p>0.05). Uji ANOVA satu jalan menunjukkan adanyaperbedaan yang signifikan antar variabel (p<0.05). Uji Post-Hoc LSDmenunjukkan adanya perbedaan yang bermakna antar konsentrasi. Hasil penelitianini menunjukkan bahwa ekstrak kulit manggis dapat menghambat pertumbuhanbiofilm Treponema denticola ATCC 35405. Pada masa inkubasi 1 dan 3 jam,konsentrasi ekstrak kulit manggis 25% memiliki kemampuan yang setaraklorheksidin dan tidak berbeda bermakna. Pada masa inkubasi 24 jam, konsentrasi25% lebih efektif dan berbeda bermakna dengan klorheksidin. Ekstrak kulitmanggis dengan konsentrasi 25% pada seluruh masa inkubasi terbukti paling efektifdiantara seluruh konsentrasi pada penelitian ini.

P eriodontal disease is one of the most oral health problem found in Indonesia. Theetiology that causes periodontal disease is plaque. One of the bacteria in plaque thatplays an important role in the pathogenesis of periodontal disease is Treponemadenticola. Mangosteen (Garcinia mangostana L.) which is proven to haveantibacterial, antioxidant, and anti-inflammatory properties. This study aimed todetermine the effect of mangosteen (Garcinia mangostana L.) peel extract on thedensity of Treponema denticola ATCC 35405 biofilm. Mangosteen was obtainedfrom the Balittro Laboratory in Bogor, West Java. This research was held at theMicore Laboratory of Faculty of Dentistry Trisakti University. Treponemadenticola were cultured in 96 well-plates for 1x24 hours then mangosteen peelextract was dropped with a concentration of 100%, 50%, 25%, 12.5%, 6.25%, BrainHeart Infusion (BHI) broth (negative control) medium and chlorhexidine 0,2%(positive control). The well-plate was incubated for 1 hour, 3 hours, and 24 hoursat a temperature of 37Â° and then given a crystal violet staining and read using amicroplate reader. The normality test using the Shapiro-Wilk method shows thatthe data is normally distributed (p> 0.05). One-way ANOVA test showed asignificant difference between variables (p <0.05). The Post-Hoc LSD test showeda significant difference between concentrations. The results of this study indicatethat mangosteen peel extract can inhibit the growth of Treponema denticola ATCC35405 biofilms. During the incubation period of 1 and 3 hours, the concentration ofmangosteen peel extract 25% has an equivalent ability of chlorhexidine and notsignificantly different. During the 24-hour incubation period, the concentration of25% was more effective and significantly different from chlorhexidine.Mangosteen peel extract with a concentration of 25% during the entire incubationperiod proved to be the most effective of all concentrations in this study.