Efek minyak atsiri cendana india (santalum album l.) terhadap pertumbuhan dan biofilm fusobacterium nucleatum dan treponema denticola (in vitro)

DETAIL KOLEKSI

Efek minyak atsiri cendana india (santalum album l.) terhadap pertumbuhan dan biofilm fusobacterium nucleatum dan treponema denticola (in vitro)

Oleh : Clarissa Valeri

Info Katalog

Nomor Panggil : 616.904 1 CLA e

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2024

Pembimbing 1 : Ciptadhi Tri Oka Binartha

Subyek : Microbiology - Periodontal disease

Kata Kunci : Indian sandalwood (Santalum album L.) essential oil, biofilm, Fusobacterium nucleatum, Treponema den

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.
1.	2024_TA_SKG_040002000100_Halaman-Judul.pdf	12
2.	2024_TA_SKG_040002000100_Lembar-Pengesahan.pdf	4
3.	2024_TA_SKG_040002000100_Bab-1_-Latar-Belakang.pdf	4
4.	2024_TA_SKG_040002000100_Bab-2_-Tinjauan-Pustaka.pdf	13
5.	2024_TA_SKG_040002000100_Bab-3_Kerangka-Teori,-Kerangka-Konsep,-dan-Hipotesis.pdf	3
6.	2024_TA_SKG_040002000100_Bab-4-Metode-Penelitian.pdf	12
7.	2024_TA_SKG_040002000100_Bab-5_Hasil-Penelitian.pdf	22
8.	2024_TA_SKG_040002000100_Bab-6_Pembahasan.pdf	4
9.	2024_TA_SKG_040002000100_Bab-7_Kesimpulan-dan-Saran.pdf	1
10.	2024_TA_SKG_040002000100_Daftar-Pustaka.pdf	13
11.	2024_TA_SKG_040002000100_Lampiran.pdf	22

Abstrak
Abstract

L atar belakang: Penyakit periodontitis disebabkan bakteri pathogencontohnya Fusobacterium nucleatum dan Treponema denticola.Periodondititis dapat dicegah dengan kontrol plak kimiawi menggunakanklorheksidin, namun pemakaian klorheksidin berlebihan menyebabkanperubahan warna pada gigi. Ekstrak minyak atsiri cendana India merupakanbahan alam yang dapat digunakan sebagai alternatif mempunyai fungsimenghambat pertumbuhan bakteri. Tujuan: Untuk mengetahui efek minyakatsiri cendana India dalam menghambat pertumbuhan dan biofilmFusobacterium nucleatum dan Treponema denticola. Metode: Bakteri F.nucleatum dan T. denticola dikultur pada suhu 37Â°C selama 24 jam. Minyakatsiri 100% dilarutkan menjadi konsentrasi 50%, 25%, 12,5%, 6,25%,3,125%, 1,56%. Uji antimikroba dilakukan menggunakan metode mikrodilusidengan menghitung rerata koloni. Uji biofilm dilakukan dengan metodebiofilm assay dengan masa inkubasi 1, 3, dan 24 jam. Hasil uji biofilm dilihatdengan microplate reader dengan panjang gelombang 490 nm. Hasil:Mikrodilusi F. nucleatum dan T. denticola didapat daya hambat minimal dikonsentrasi 50%. Uji biofilm F. nucleatum didapat daya hambat dikonsentrasi 50% masa inkubasi 1 jam, sedangkan dapat dibunuh dikonsentrasi 50% masa inkubasi 3 jam dan di konsentrasi 50%, 25%, 12,5%,6,25% masa inkubasi 24 jam. Uji biofilm T. denticola didapat daya hambat dikonsentrasi 50% masa inkubasi 1 dan 3 jam, sedangkan dapat dibunuh dikonsentrasi 50%, 25%, 12,5%, 6,25% masa inkubasi 24 jam. Kesimpulan:Ekstrak minyak atsiri cendana India terbukti efektif menghambatpertumbuhan dan biofilm F. nucleatum dan T. denticola.

B ackground: Periodontitis caused by pathogenic bacteria such asFusobacterium nucleatum and Treponema denticola. Periodontitis preventedby chemical plaque control using chlorhexidine, but excessive chlorhexidinecauses discoloration of the teeth. Indian sandalwood essential oil extract is anatural ingredient can be used as an alternative and have function inhibitingbacterial growth. Purpose: To determine the effect of Indian sandalwoodessential oil in inhibiting the growth and biofilm of Fusobacterium nucleatumand Treponema denticola. Method: F. nucleatum and T. denticola bacteriacultured at 37Â°C for 24 hours. The 100% essential oil dissolved intoconcentrations 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%. Antimicrobial testsusing microdilution method by calculating average colonies. Biofilm testusing biofilm assay method with incubation period 1, 3, and 24 hours.Biofilm assay results viewed with a microplate reader with a wavelength 490nm. Results: Microdilution of F. nucleatum and T. denticola obtainedminimal inhibition at 50% concentration. Fusobacterium nucleatum biofilmtest obtained inhibition at 50% concentration for 1 hour incubation period,while it can be killed at 50% concentration for 3 hours incubation and at 50%,25%, 12,5%, 6,25% concentration for 24 hours incubation. Treponemadenticola biofilm test obtained inhibition at 50% concentration for 1 and 3hours incubation, while it can be killed at 50%, 25%, 12,5%, 6,25%concentration for 24 hours incubation. Conclusion: Indian sandalwoodessential oil extract proved effective in inhibiting the growth and biofilm ofF. nucleatum and T. denticola.