Ukuran dan granularitas sel punca pulpa molar ketiga yang ompaksi (laporan penelitian)
S el punca telah banyak diteliti dan digunakan dalam bidang kedokteran regeneratif. Sel punca merupakan sel yang belum berdiferensiasi, mampu memperbanyak diri dan berdiferensiasi. Sel tersebut dapat ditemukan di berbagai macam jaringan salah satunya adalah pulpa gigi. Dengan kemampuan diferensiasi, sel punca dapat digunakan untuk meregenerasi jaringan yang rusak. Peneliti di Indonesia telah mengisolasi dan mengkultur sel punca pulpa dari gigi molar ketiga yang impaksi (SPPM3). Melalui penelitian tersebut diketahui bahwa SPPM3 memiliki kemampuan osteogenesis dan mengekspresikan cluster of differentiation (CD)117. Akan tetapi penelitian tersebut tidak melaporkan morfologi SPPM3, yaitu ukuran dan granularitas sel. Pada penelitian ini, ukuran dan granularitas SPPM3 diperiksa menggunakan flow cytometer. Pertama-tama dilakukan thawing dan kultur SPPM3, lalu dilanjutkan dengan pemisahan SPPM3. Kemudian SPPM3 dimuatkan pada FACSCanto II yang dilanjutkan dengan analisa hasil gating. Kedua parameter yaitu forward scatter (FSC) dan side scatter (SSC) ditampilkan dalam scatter plot dan dianalisis lebih lanjut. Parameter FSC menunjukkan ukuran sel, sedangkan SSC menunjukkan granularitas dari sel. Hasil analisis flow cytometry menunjukkan bahwa ukuran SPPM3 berkisar dari 75 sampai 230 dan granularitas SPPM3 berkisar dari 27 sampai 203. Ukuran dan granularitas SPPM3 berbeda dibandingkan dengan SPM dari jaringan-jaringan lain. Ukuran dan granularitas SPM dari setiap sumber adalah berbeda. Perbedaan ini disebabkan adanya perbedaan ekspresi marker SPM dan ekspresi senescenceassociated marker.
S tem cells have been investigated and used in regenerative medicine. Stem cells are undifferentiated cells with proliferation and differentiation ability. These cells can be found in various tissues such as the dental pulp. With their differentiation capabilities, stem cells can be used to regenerate damage tissues. Researchers in Indonesia isolated and cultured the dental pulp stem cell from the impacted third molar (DPSC-M3). The study showed that DPSC-M3 have osteogenesis potential and express CD117. However, the size and granularity of DPSC-M3 have not been investigated. Thus, this research was conducted to investigate the size and granularity of DPSC-M3. In this research, the size and granularity were examined using flow cytometer. Initially the cells were thawed, cultured and separated. Afterwards the cells were loaded into FACSCanto II and gating results were analysed. There were two parameters measured and presented in scatter plot, forward scatter (FSC) and side scatter (SSC). The FSC showed the cell’s size while SSC showed the cell’s granularity. The results of this research showed that DPSC-M3’s size were ranging from 75 to 230, while the granularity were from 27 until 203. The size and granulation of DPSC-M3 were different compared to the ones of mesenchymal stem cell (MSC) from other tissues. These variations were caused by difference in each MSC expression and senescence-associated marker expression.