S eptic arthritis Temporo Mandibular Joint (TMJ) is one of the complications post tooth molar extraction that possibly extends to the joint, based on research two bacterias considered as a pivotal factor can be found in the aspiration of joint fluid are Staphylococcus aureus and Streptococcus pyogenes. Early prevention and treatment are needed, until these days people are still choosing chemical drugs over herbal drugs. Jamblang plant (Syzygium cumini) is a traditional herbal plant that has useful content, part of this plant that is often used medicine are stem, rind, fruit, seed, and leaf. Previous research has proven the benefits of this plant as antifungal, antiviral, and Gram positive-negative antibacterial. Leaf is one of the parts of the plant that is affordable to obtain. This study aim to identify the inhibiton of jamblang' s leaf extract on bacteria Staphylococcus aureus and Streptococcus pyogens cause of Septic Arthritis TMJ in vitro. This research is done through an experimental laboratory using difussion of wells method, each hole was filled with different concentration of jamblang's leaf extract (100%, 50%, 25%, 12,5%, 6,25%, 3,125%), chlorhexidine 0,2% as a positive control, and aquades as a negative control. The plates were incubated at 37°C for lx24 hours. The results of this study on both type of bacteria shown the formation of zone inhibition were present around each concentration group but has a different diameter. Statistic analysis of this study used one-way Anova, it can be concluded that jamblang's leaf extract has growth-inhibiting effect against Staphylococcus aureus and Streptococcus pyogenes (p < 0,05>, followed by Tukey HSD. The results in this study shows jamblang's leaf extract 3,125% inhibit bacteria Staphylococcus aureus and Streptococcus pyogenes, but it has low effectiveness.