B Background: The dental pulp is a vital tissue protected within the dental dentin containing nerves, blood vessels, connective tissue, and various types of cells. Pulpitis is the inflammatory response of tooth pulp to common dental injury. The use of stem cells, scaffolds, and growth factors can regenerate the pulp-dentin complex and restore tooth function in the tooth regeneration process. Natural substances can serve as alternatives in the search for materials capable of regenerating pulp cells, such as Alstonia scholaris or Pulai. These plants have various effects, including anti-inflammatory, analgesic, antibacterial, anticancer, antifungal properties, as well as accelerating wound healing. Objective: To investigate the effect of A. scholaris leaf extracts on the viability and migration speed of dental pulp stem cells in vitro. Methods: Dental pulp stem cells were treated with methanol extract of A. scholaris leaf(l 0 µg/mL, 50 µg/mL, 200 µg/mL, and 1,000 µg/mL) for 24 and 48 hours. Cell viability was analyzed using the MTT assay, and migration speed was assessed through the scratch wound healing assay. Statistical analysis was performed. Results: A. scholaris leaf extracts was able to enhance the viability and migration of dental pulp stem cells at concentrations of 10 and 50 µg/mL. Conclusion: A. scholaris leaf extracts significantly improves the viability and migration of dental pulp stem cells at a concentration of 50 µg/mL.