Perbandingan ekspresi SOD 1 pada variasi Genomik rs2234694 Gen SOD1 penderita kanker serviks

DETAIL KOLEKSI

Perbandingan ekspresi SOD 1 pada variasi Genomik rs2234694 Gen SOD1 penderita kanker serviks

Oleh : Muhammad Ibnu Mundzir Sarman

Info Katalog

Nomor Panggil : S 2070

Penerbit : FK - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2023

Pembimbing 1 : Monica Dwi Hartanti

Subyek : Superoxide dismutase;Cervix uteri--Cancer

Kata Kunci : Superoxide dismutase-1, polymorphism, cervical cancer, gene expression

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.
1.	2023_TA_SKD_030001900088_Lembar-Halaman.pdf	15
2.	2023_TA_SKD_030001900088_Pengesahan.pdf	1
3.	2023_TA_SKD_030001900088_BAB-1_Pendahuluan.pdf	5
4.	2023_TA_SKD_030001900088_BAB-2_Tinjauan-Literatur.pdf	19
5.	2023_TA_SKD_030001900088_BAB-3_Kerangka-Teori.pdf	3
6.	2023_TA_SKD_030001900088_BAB-4_Metode-Penelitian.pdf	12
7.	2023_TA_SKD_030001900088_BAB-5_Hasil.pdf	3
8.	2023_TA_SKD_030001900088_BAB-6_Pembahasan.pdf	6
9.	2023_TA_SKD_030001900088_BAB-7_Kesimpulan.pdf	1
10.	2023_TA_SKD_030001900088_Daftar-Pustaka.pdf	7
11.	2023_TA_SKD_030001900088_Lampiran.pdf	19

Abstrak
Abstract

K anker serviks disebabkan oleh Human Papilloma Virus (HPV), denganmengintegrasikan materi genetiknya ke dalam sel serviks. Perubahan level ROSmenyebabkan perubahan antioksidan tubuh untuk mencegah terjadinya stresoksidatif. Terjadinya variasi pada gen SOD1 diperkirakan menyebabkan kerusakanenzim karena ekspresi gen yang terganggu, khususnya titik rs2234694 sehinggamenyebabkan akumulasi superoksida beracun. Maka, perlu dilakukan analisauntuk membandingkan ekspresi gen variasi genomik SOD1 rs2234694.METODEPenelitian ini merupakan penelitian analitik observasional biomolekuler denganpendekatan cross-sectional pada 31 wanita penderita kanker serviks, variasigenomik dianalisis dengan metode RFLP dan eskpresi gen dianalisis menggunakanmetode qRT-PCR. Apabila dideteksi variasi genomik maka dilakukan uji Kruskal-Wallis.HASILDitemukan pasangan alel AA pada seluruh subjek pada wanita dengan kankerserviks sedangkan pasangan alel CC dan AC tidak ditemukan sama sekali, sehinggatidak dapat dilakukan uji statistik untuk menguji perbandingan ekspresi gen SOD1pada variasi genomik SOD1 rs2234594. Tidak ditemukan perbedaan bermaknapada ekspresi gen SOD1 antar stadium kanker serviks dengan (p-value:0,846)KESIMPULAN DAN SARANTidak terdapat adanya variasi genomik SOD1 rs2234694 pada wanita penderitakanker serviks. Variasi genomik SOD1 rs2234694 tidak mempunyai peran besardalam terjadinya kanker serviks. Disarankan dilakukan penelitian pada titik variasigen SOD1 yang lain atau pada antioksidan endogen lainnya

C ervical cancer is caused by the Human Papilloma Virus (HPV), by integrating itsgenetic material into cervical cells. Changes in ROS levels cause changes in thebody's antioxidants to prevent oxidative stress. The occurrence of variations in theSOD1 gene is thought to cause enzyme damage due to disturbed gene expression,especially the rs2234694 point, causing the accumulation of toxic superoxide.Thus, it is necessary to carry out an analysis to compare the gene expression of thegenomic variation of SOD1 rs2234694.METHODThis research is a biomolecular observational analytic study with a cross-sectionalapproach in 31 women with cervical cancer, genomic variation was analyzed usingthe RFLP method and gene expression was analyzed using the qRT-PCR method.If genomic variation is detected, the Kruskal-Wallis test is performed.RESULTSAA allele pairs were found in all subjects in cervical cancer women, while CC andAC allele pairs were not found at all, so statistical tests could not be performed tocompare SOD1 gene expression in the SOD1 rs2234594 genomic variation. Nosignificant differences were found in SOD1 gene expression between stages ofcervical cancer with (p-value: 0.846)CONCLUSIONS AND SUGGESTIONSThere were no genomic variations of SOD1 rs2234694 in patients with cervicalcancer. SOD1 rs2234694 genomic variation does not have a major role in theoccurrence of cervical cancer. It is recommended to do research on other SOD1gene variation points or on other endogenous antioxidants.