C ervical cancer is a neoplastic malignancy in the cervical epithelium. Humanpapillomavirus (HPV) infection is a cause of cervical cancer. Recent researchsuggests the integration of HPV with the Semaphorin 3D (SEMA3D) gene thattriggers cervical cancer. However, the research is in vitro and research in vivo isneeded. Given the important role of HPV infection in cervical cancer, the SEMA3Dgene expression research was conducted.METHODSThe design of this study uses observational analytic methods with cross sectionalapproach. Samples were taken by consecutive non-random sampling in periodNovember - January 2020. Samples were taken by doing pap smear and tissuebiopsy at Trisakti Medical Center. Samples were examined for gene expressionusing the qPCR technique at the Molecular Biology Laboratory. Data analysis usingIndependent Sample t test on GraphPad Prism8 for Mac.RESULTSIn this study, the average value of SEMA3D are 6 gene expression in normalcervical cell samples was 0.31644114 and in 4 cervical cancer cells 0.21641108.Independent Sampel T-Test statistical test results there was no significant averagedifference in comparison of SEMA3D gene expression between normal cervical celland cervical cancer cells. Have a value of p=0,5854 (p>0,05).CONCLUSSIONThis study shows the SEMA3D gene expression wasn’t significantly different in thecervical cancer group and the normal group. The possibility of the SEMA3D genedoes not play an active role in the process of proliferation and migration of cervicalcancer and there is a role of other genes.