T The Cymbopogon citratus, commonly known as the citronella plant, hasgained significant attention as a potential source of natural ingredients for the developmentof medicinal products. This plant species contains a range of biologically activecompounds such as neral, citral, geranial acetate, flavonoids, and tannins, which possessa variety of pharmacological activities such as antibacterial, antifungal, and antiinflammatory.Previous studies have demonstrated the antibacterial properties ofcitronella leaf extract and chitosan against diverse bacterial strains. Notably,Staphylococcus aureus and Streptococcus pyogenes are among the types of bacteria thatcould potentially cause oral infections in individuals with poor hygiene. Objective: Theobjective of this study is to evaluate the inhibitory potential of citronella leaf extract thathas been encapsulated with chitosan against Staphylococcus aureus and Streptococcuspyogenes bacteria. Method: Experimental studies use diffusion discs to determine theinhibitory zone of each encapsulated preparation. The test group was divided into fourchitosan comparison preparations: NaTPP, which is 1:1; 1:0,9; 1:0,8; 1:0,7. Results: Thepresent study investigated the inhibitory zone against two types of bacteria, S. aureus andS. pyogenes, for various encapsulation ratios. The results showed that the inhibitory zoneof S. aureus bacteria for each encapsulation ratio, 1:1; 1:0.9; 1:0.8; 1:0.7, was 25.09±0.62mm, 21.01±0.13 mm, 22.78±0.39 mm, and 23.96±0.14 mm, respectively. Similarly, theinhibition zone against the growth of S. pyogenes bacteria was observed for the sameencapsulation ratios, namely 1:1; 1:0.9; 1:0.8; and 1:0.7. The corresponding inhibitoryzones were 24.38±0.45 mm, 21.99±0.34 mm, 22.44±0.48 mm, and 20.49±0.24 mm,respectively. These findings suggest that the encapsulation ratio has a significant impacton the inhibitory efficacy of the tested bacteria. Further research is warranted to explorethe optimal encapsulation ratio for maximum inhibitory activity against pathogenicbacteria. Conclusion: Citronella leaf extract effectively inhibits the growth of S.aures andS.pyogenes bacteria, with a concentration ratio of 1:1 shown to have the highestantibacterial effect.