M Mouthwash is a solution or a liquid used to rinse the mouth with a number of objectives, among others, to get rid of bacteria destroyer, eliminate the odor, has a therapeutic effect and eliminate the infection or prevent dental caries. Chitosan is a natural carbohydrate biopolymers which are derivatives of chitin by deacetylation process that acts as an antibacterial agent. The purpose of this study was to determine the nano chitosan and calcium based mouthwash formulation from beetle horns exoskeleton are most preferred by the panelists (Faculty of Dentistry Trisakti) through organoleptic assessment studies using hedonic test (Test A). Organoleptic test was conducted at 30 panelists with different mouthwash formulation of 250 ppm, 500 ppm and 750 ppm. The study states that the level of acceptance of mouthwash with a preferred concentration of 500 ppm (93.3%) in terms of viscosity, mouthwash with a preferred concentration of 250 ppm (93.3%) in terms of aroma, mouthwash with a concentration of 250 ppm and preferably 500 ppm (100%) in terms of clarity, and mouthwash with a preferred concentration of 500 ppm (86.7%) in terms of taste