B Background: According to the World Health Organization (WHO), in 2016, severe periodontal disease causing tooth loss was ranked 11th in the world. Periodontitis is usually caused by Gram-negative bacteria colonizing subgingival plaque, one of them is P.gingivalis. Other than mechanical treatment, the use of mouthwash and antibiotics are carried out by using natural ingredients such as P. amboinicus leaf extract which has antibacterial effects. Purpose: This study aims to determine the difference in the effect of the ethanol extract and the ethyl acetate extract of P. amboinicus leaves on the growth of P. gingivalis. Method: This study uses the disk-diffusion method with P. amboinicus extract, fractions of hexane, ethyl acetate and ethanol as test materials for P.gingivalis cultures. Then, aquadest as negative control, while chlorhexidine and tetracycline as positive control. Result: The result of this study shows that at 24 hours measurement, it can be seen that the average diameter of the inhibition zone for the ethanol fraction with a concentration of 25%, 50% and 75% (mg/ml) is 10.2 mm, 12.017 mm, and 13.017 mm. The average diameter of the inhibition zone for the ethyl acetate fraction with a concentration of 25%, 50% and 75% (mg/ml) were 10.05 mm, 11.583 mm, and 12.133 mm. Conclusion: The ethanol fraction and ethyl acetate of P. amboinicus leaves can inhibit the growth of P.gingivalis. The data obtained showed that the inhibition zone produced by the ethanol extract was greater than that of the ethyl acetate extract.