I nstallation of a Domestic Wastewater Treatment Plant (STP) is a specialized engineering structure and equipment, along with its complements, explicitly designed to treat wastewater from household activities. Wastewater treatment involves several stages, including pre-treatment, primary, and advanced treatment. One alternative advanced wastewater treatment system is the Subsurface Constructed Wetland and Multilayer filtration (SCW-MLF) method, which utilizes several layers of filter media with different particle sizes dominated by zeolite. The research was conducted at IPAL Krukut to reduce organic matter in the wastewater effluent from the equalization tank. The objective of this study was to test the effectiveness of the SCW-MLF reactor in removing N (nitrogen) and P (phosphorus) with variations in retention time (HRT). The study also aimed to calculate the areal removal rate constant (kA) and the volumetric removal rate constant (kV). The retention time variations were 48, 36, 24, 18, and 12 hours. The results showed removal efficiencies of ammonia ranging from 50% to 98%; TN (total nitrogen) from 74% to 88%; TP from 43% to 90%; and oils and grease from 69.93% to 97.33%. The values of the areal removal rate constant (kA) obtained in this research were as follows: for ammonia, it ranged from 0.2 to 3.5 m/day; for TN, it ranged between 0.37 to 1.3 m/day; for TP, it ranged between 0.24 to 0.93 m/day; and for oils and fats, it ranged between 0.68 to 0.99 m/day. The values of volumetric removal rate constants (kV) range from 0.457 to 8.393 /day for ammonia; 0.89 to 3.15 /day for TN (total nitrogen); 0.585 to 2.267 /day for TP and 1.659 to 2.402 /day for oil and grease parameter. The results demonstrated that the performance of the subsurface constructed wetland multilayers filtration zeolite with the presence of fragrant root plants (V. zizanioides) in treating effluent met the domestic wastewater quality standards as stated in PerMen LHK No.68 of 2016 and reduced stench from the wastewater effluent.