Pengaruh ekstrak daun alstonia scholaris terhadap proliferasi sel punca pulpa gigi

DETAIL KOLEKSI

Pengaruh ekstrak daun alstonia scholaris terhadap proliferasi sel punca pulpa gigi

Oleh : Fatwanissa Ariestha Putri

Info Katalog

Nomor Panggil : 612.015 FAT p

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2024

Pembimbing 1 : Muhammad lhsan Rizal

Pembimbing 2 : Ria Aryani Hayuningtyas

Subyek : Biochemistry - Biomedicine

Kata Kunci : alstonia scholaris, viability, migration, dental pulp stem cells.

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.
1.	2024_TA_SKG_040002000134_Halaman-Judul.pdf	12
2.	2024_TA_SKG_040002000134_Lembar-Pengesahan.pdf	3
3.	2024_TA_SKG_040002000134_Bab-1_Pendahuluan.pdf	4
4.	2024_TA_SKG_040002000134_Bab-2_Tinjauan-Pustaka.pdf	12
5.	2024_TA_SKG_040002000134_Bab-3_Kerangka-Teori.pdf	4
6.	2024_TA_SKG_040002000134_Bab-4_Metode-Penelitian.pdf	10
7.	2024_TA_SKG_040002000134_Bab-5_Hasil.pdf	5
8.	2024_TA_SKG_040002000134_Bab-6_Pembahasan.pdf	2
9.	2024_TA_SKG_040002000134_Bab-7_Kesimpulan-dan-Saran.pdf	1
10.	2024_TA_SKG_040002000134_Daftar-Pustaka.pdf	8
11.	2024_TA_SKG_040002000134_Lampiran.pdf	20

Abstrak
Abstract

L Latar Belakang: Pulpa gigi adalah jaringan vital yang terlindungi di dalam dentin gigi berisi saraf, pembuluh darah, jaringan ikat, dan berbagai macam sel. Pulpitis adalah respon inflamasi pulpa gigi terhadap kerusakan gigi yang umum terjadi. Penggunaan stem cell, scaffold, dan growth factor dapat meregenerasi kompleks dentin pulpa dan memulihkan fungsi gigi dalam proses regenerasi gigi. Bahan alam dapat menjadi alternatif dalam mencari bahan yang dapat meregenerasi sel pulpa, seperti Alstonia scholaris atau Pulai. Tumbuhan ini memiliki berbagai efek berupa anti-inflamasi, analgesik, antibakteri, antikanker, antifungal, serta mempercepat penyembuhan Iuka. Tujuan: Untuk mengetahui pengaruh ekstrak daunA. scholaris terhadap viabilitas dan kecepatan migrasi sel punca pulpa gigi secara in vitro. Metode: Sel punca pulpa gigi diberikan perlakuan dengan esktrak metanol daun A. scholaris (10 Âµg/mL, 50 Âµg/mL, 200 Âµg/mL dan 1.000 Âµg/mL) selama 24 dan 48 jam. Viabilitas sel dianalisis dengan uji MTT dan kecepatan migrasi dengan uji scratch wound healing. Analisis statistik dilakukan. Hasil: Ekstrak daun A. scholaris mampu meningkatkan viabilitas dan migrasi sel punca pulpa gigi pada konsentrasi 10 dan 50 Âµg/mL. Kesimpulan: Ekstrak daun A. scholaris mampu meningkatkan viabilitas dan migrasi sel punca pulpa gigi secara signifikan pada konsentrasi 50 Âµg/mL.

B Background: The dental pulp is a vital tissue protected within the dental dentin containing nerves, blood vessels, connective tissue, and various types of cells. Pulpitis is the inflammatory response of tooth pulp to common dental injury. The use of stem cells, scaffolds, and growth factors can regenerate the pulp-dentin complex and restore tooth function in the tooth regeneration process. Natural substances can serve as alternatives in the search for materials capable of regenerating pulp cells, such as Alstonia scholaris or Pulai. These plants have various effects, including anti-inflammatory, analgesic, antibacterial, anticancer, antifungal properties, as well as accelerating wound healing. Objective: To investigate the effect of A. scholaris leaf extracts on the viability and migration speed of dental pulp stem cells in vitro. Methods: Dental pulp stem cells were treated with methanol extract of A. scholaris leaf(l 0 Âµg/mL, 50 Âµg/mL, 200 Âµg/mL, and 1,000 Âµg/mL) for 24 and 48 hours. Cell viability was analyzed using the MTT assay, and migration speed was assessed through the scratch wound healing assay. Statistical analysis was performed. Results: A. scholaris leaf extracts was able to enhance the viability and migration of dental pulp stem cells at concentrations of 10 and 50 Âµg/mL. Conclusion: A. scholaris leaf extracts significantly improves the viability and migration of dental pulp stem cells at a concentration of 50 Âµg/mL.