Efektivitas antibakteri dan antibiofilm ekstrak nigella sativa dan clinacanthus nutans terhadap treponema denticola: Penelitian in vitro dan ex vivo
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Pembimbing 3 : M. Orliando Roeslan
Nomor Panggil : 617.634 2 NOV e
Subyek : Prosthodontics
Penerbit : FKG - Usakti
Kota Terbit : Jakarta
Tahun Terbit : 2023
Pembimbing 1 : Meiny Faudah Amin
Pembimbing 2 : Selviana Wulansari
Kata Kunci : Nigella sativa, Clinacanthus nutans, Treponema denticola, Root canal infection, Endodontic therapy
Status Posting : Published
Status : Lengkap
File Repositori
| No. | Nama File | Hal. | Link |
|---|---|---|---|
| 1. | 2023_TS_KG_114402010007_Halaman-judul.pdf | 14 | |
| 2. | 2023_TS_KG_114402010007_Lembar-pengesahan.pdf | 7 | |
| 3. | 2023_TS_KG_114402010007_Bab-1-Pendahuluan.pdf | 4 | |
| 4. | 2023_TS_KG_114402010007_Bab-2-Tinjauan-pustaka.pdf | 12 |
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| 5. | 2023_TS_KG_114402010007_Bab-3-Kerangka-teori,-konsep,-dan-hipotesis.pdf | 4 |
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| 6. | 2023_TS_KG_114402010007_Bab-4-Metode-penelitian.pdf | 20 |
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| 7. | 2023_TS_KG_114402010007_Bab-5-Hasil-penelitian.pdf | 8 |
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| 8. | 2023_TS_KG_114402010007_Bab-6-Pembahasan.pdf | 3 |
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| 9. | 2023_TS_KG_114402010007_Bab-7-Kesimpulan-dan-saran.pdf | 2 |
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| 10. | 2023_TS_KG_114402010007_Daftar-pustaka.pdf | 5 | |
| 11. | 2023_TS_KG_114402010007_Lampiran.pdf | 13 |
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L Latar Belakang: Prevalensi Treponema denticola adalah yang tertinggi di antara patogen utama dalam periodontitis apikal. Nigella sativa dan Clinacanthus nutans merupakan tanaman herbal yang memiliki banyak efek terapeutik. N. sativa mengandung thymoquinone, dan C. nutans mengandung flavonoid, saponin, dan fenol, yang diyakini memiliki sifat antibakteri dan antibiofilm dengan efek samping minimal. Tujuan: Untuk mengetahui aktivitas antibakteri dan antibiofilm dari ekstrak biji N. sativa dan daun C. nutans terhadap T. denticola. Metode: Penelitian ini menggunakan ekstrak etanol biji N. sativa dan daun C. nutans dalam berbagai konsentrasi: 100%, 50%, 25%, 12,5%, 6,25%, dan 3,125%, Natrium hipoklorit 5,25% digunakan sebagai kontrol positif dan aquades digunakan sebagai kontrol negative. T. denticola ATCC 35405 dikultur selama 24 jam. Konsentrasi Hambat Minimum ditentukan dengan metode difusi sumuran agar. Aktivitas antibiofilm ditentukan dengan metode uji biofilm dengan pewarnaan kristal violet selama 1, 3 dan 24 jam. RT-PCR kuantitatif dilakukan untuk mengevaluasi tingkat ekspresi gen dalam T. denticola. Hasil: Ekstrak N. sativa dan C. nutans tidak menunjukkan aktivitas antibakteri terhadap T. denticola. Semua konsentrasi ekstrak N. sativa dan C. nutans dalam inkubasi selama 24 jam ditemukan memiliki aktivitas antibiofilm terhadap T. denticola. Kesimpulan: Ekstrak N. sativa dan C. nutans mengalami pengurangan biofilm, menunjukkan potensi mereka dalam eradikasi biofilm T. denticola.
B Background: The prevalence of Treponema denticola is the highest among the key pathogen associated with apical periodontitis.Nigella sativa and Clinacanthus nutans are herbal plants that have many therapeutic effects. N. sativa contains thymoquinone, and C. nutans contains flavonoids, saponins, and phenols, which are supposed to have antibacterial and antibiofilm properties with minimal side effects. Objectives: To determine antibacterial and antibiofilm activity of N. sativa seeds and C. nutans leaf extract on T. denticola. Methods: This study used N. sativa seeds and C. nutans leaf ethanol extracts in different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%, Sodium hypochlorite 5.25% was used as the positive control, and aquades was used as the negative control. Reference strains of T. denticola ATCC 35405 were grown for 24 hours. Minimum Inhibitory Concentration (MIC) was determined by agar well diffusion method. Antibiofilm activity was determined by the biofilm assay method with crystal violet staining for 1, 3 and 24 hour. Quantitative RT-PCR was performed to evaluate the expression levels of genes in T. denticola. Results: N. sativa and C. nutans extracts demonstrated no antibacterial activity against T. denticola. All concentrations of N. sativa and C. nutans extracts in a 24-hour incubation were found to exhibit antibiofilm activity against T. denticola. Conclusion: N. sativa and C. nutans extracts exhibited reduction in biofilm, indicating their potential for eradicating T. denticola biofilms.
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