T he healing of defected on the bone, was influenced by several things including smoking habits. The contents that included in cigarette could be suspected to inhibit hematoma phase and led to the increased number of leukocytes. The purpose of this study was to investigate the effects of cigarette smoke exposure after the application of bone graft by the number of leukocytes. Blood samples of control and treatment groups on the 3rd, 7th, and 14th day were taken via intracardiac.The results of the non-parametric Mann-Whitney Test day 7th concluded that there were significant differences between treatment and control groups (p = 0,001). Whereas on day 3rd and 14th concluded that there were no significant differences between treatment and control groups (p = 0,564 and p = 0,286).This study showed that there was an association between cigarette smoke exposure after the application of bone graft and the total number of leukocytes. Cigarette smoke exposure after the application of bone graft was also proven to be the cause of increase the number of leukocytes.