T This research purposes to removal and recovery of copper (Cu2+) heavy metals with a biotechnological approach using mixed microalgae Chlorella sorokiniana, Monoraphidium sp. and Scenedesmus obliquus which is bound in Na-Alginate polymer. The effect of temperature and contact time on the biosorption and desorption process was examined to achieve the greatest efficiency in the Cu2+ metal removal. Immobilized biosorbents are made by drying microalgae biomass in the oven, grinding, and trapping into alginate polymers to form immobilized biosorbents or called beads. The reduction and release of Cu concentration was analyzed using AAS. The effectiveness of biosorbents on biosorption and desorption processes was analyzed using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). Optimization of biosorption and desorption was carried out in a batch system using erlenmeyer in shaker containing a concentration solution of 18.266 mg/L Cu2+, biosorbent 0.5 g/g, pH 4, rotation speed 180 rpm, with temperature regulation (25, 35, 45) oC and contact time (60, 120, 180) minute. The results showed that maximum biosorption occurred at 35oC for 120 minutes with removal efficiency reaching 98.96%. Biosorbent is able to release Cu2+ heavy metals using 0.1 N HCl as desorbent solution at 35oC for 60 minutes with a release efficiency of 76.11%. The functional groups of carboxylic acids, sulfihydriles, ketones, amides, amines, aromatics, alkanes and alkenes are proven to be inside the biosorbent cell walls. The results of the FTIR analysis also provided information that the amides, ketone, and sulfihydriles functional groups were the most dominant during the biosorption and desorption processes. The morphology of biosorbents was damaged after biosorption and desorption processes which is characterized by cracks on the biosorbent surface which had been demonstrated using SEM. The adsorption process of this study follows the Langmuir Isoterm equation and pseudo-second-order with a linear regression coefficient (R2) which is closest to the number 1, that is equal to 0.999. This study proves that immobilized biosorbent is very effective for reducing Cu2+ concentration in wastewater which is indicated by the absorption efficiency more than 50% and can be reused as biosorbent, so that it can be an alternative method of controlling heavy metal pollution in waters that are competitive and environmentally friendly.