O bjective: To investigate whether there is a difference between male and female MCI (Mandibular Canine Index) in Indonesia for sex screening purposes to aid in the identification process. Materials and methods: 35 study models were made from 19 female and 16 male students from the dentistry major of Trisakti University ranging from 18-25 years old. Mesiodistal dimensions of the left and right mandibular canine and intercanine distance were measured using a digital vernier caliper, followed by calculations of the left and right MCI. These values were subjected to statistical analysis by using the 2 samples t-test. Results: There were indeed differences between the mandibular intercanine distance, mesiodistal width of the mandibular canine, and MCI between male and female, with male values being larger than females in all parameters. Although the mandibular intercanine distance did not show any statistically significant difference between sexes (p>0,05), the present study has established a statistically significant difference between the mesiodistal width of both the mandibular left and right canine as well as the left and right MCI between men and women in Indonesia (p<0,05). The standard MCI had an accuracy of 71.43% in sex determination, proving that sexual dimorphism could be seen in those parameters. Conclusion: From the MCI, including the mandibular intercanine distance and mesiodistal width of the mandibular canine, it could be concluded that sexual dimorphism is present in the mandibular canine teeth and that these values could be used as a quick and fairly easy method for sex determination in the Indonesian population.