B ackground: Temporomandibular joint disorder is a significant health problem.Complaints are often felt by tuba players with the long duration of exercise. Thiscan cause muscle fatigue that occur due to tension on the head muscles, mandible,and face. The duration of the exercise includes the length of active playing tuba, thenumber of training days per week, and the duration of training per day. Objective:The aim of this study was to investigate the relationship between the long durationof exercise with temporomandibular joint disorder on the tuba players in marchingband group in Jakarta and surrounding areas. Methods: Type of this research is anobservational analytic that uses cross-sectional design by giving a TMD symptomquestionnaire that has been tested for validity and reliability. Questionnaires weredistributed to tuba players who were actively playing tuba for 1 year and had nohistory of trauma to TMJ. Results: There is a significant relationship betweensymptoms of temporomandibular joint disorder with the length of active playingtuba (rs:0,446) and the number of days of exercise per week (rs:0,367). Anothersignificant relationship was found between symptom of temporomandibular jointdisorder with interpretation of tuba weight (rs:0,450). Conclusions: This studyshows the significant relationship between the long duration of exercise withtemporomandibular joint disorder on the tuba players in marching band group inJakarta and surrounding areas and exacerbated by the tuba weight.