P PT. Serasi Shipping Indonesia is a company in transport services via sea that serves the transportation of heavy equipment and four-wheeled vehicles with the primary aim of Kalimantan (Balikpapan, Samarinda, Banjarmasin). PT. Serasi Shipping Indonesia is a subsidiary of PT. Astra International with directly under the auspices of PT. Astra Logistics. Improving employee performance, supported by a suitable organizational culture or which can boost performance. This research will be carried out measurement of organizational culture and performance. Measurements using the 6 characteristics of organizational culture that is Attention to detail, Outcome orientation, People orientation, team orientation, aggressiveness, and Stability. As for the measurement of employee performance using five characteristics, namely Quality, Quantity, Timeliness, Need for supervisor, and Interpersonal impact. In the calculation of the grand mean of organizational culture and employee performance in the entire division then gained the highest value People orientation (3.694) for organizational culture and Timeliness (3.645) for the performance of employees. On the Human Resources Management division then gained the highest value People orientation (3.69) for the organizational culture and Timeliness (3.70) for the performance of employees. In the marketing division then gained the highest value People orientation (3.74) for the organizational culture and Timeliness (3.69) for the performance of employees. In the division Outcome orientation obtained the highest score (3.73) for the organizational culture and Quantity (3.63) for the performance of employees. In regression analysis Regression analysis division for Human Resources Management is as follows Y = 1294 + 0639 (OutcomeO), then to the marketing division is Y = 0241 + 0332 (AttentionTD) + 0714 (Stability), and the Division of Management Information System as follows Y = 1007 + 0683 (TEAMO). The proposal is to change given the current organizational culture of a people orientation into outcome orientation for human resources management division. In the marketing division, organizational culture people be attention to detail orientation or stability and for the management information system division organizational culture outcome orientation into team orientation. Suggestions given are the beginning of each year the employee is given a target of a supervisor for the division of human resources management . In the marketing department , the management should bring in mentors for the training of employees or management purposes complement of employees to support the work of employees . and then, the proposal for the division management information system should make the management of activities outside of work.