W Water is a natural resource that is essential for life. Water is also crucialfor the sustainability of life on Earth as it is not only consumed but also used invarious life activities. Among the available water sources, many communities relyon groundwater obtained through boreholes. Based on previous research in 2009,Jatiasih Subdistrict is classified as an area with a high to very high level ofcontamination risk, with a total Coliform count of 90,625 MPN/100 ml, whichindicates a tendency to exceed the quality standards and leads to changes ingroundwater quality. Therefore, this study aimed to assess the quality ofgroundwater in Jatiasih Subdistrict, Bekasi City, and examined the influence ofwell conditions (well depth, distance from septic tank) on the presence of totalColiform bacteria and Escherichia coli, as well as their distribution patterns.Stratified Random Sampling was used for sample collection, bacteria analysis wasconducted using the membrane filter method, and linear regression was employedto determine the relationship between well conditions and bacteria. The researchfindings revealed that the highest levels of Escherichia coli bacteria were found inthe groundwater of Jatiasih Subdistrict, with a count of 80 CFU (Colony FormingUnit)/100 ml, while the highest total Coliform count was 540 CFU/100 ml. Theseresults exceeded the standard limit of 0 CFU/100 ml. The factors contributing tobacterial contamination included the distance from the septic tank and thepollution sources. Regression analysis showed a significant relationship betweenthe distance from the septic tank, well depth, and the presence of Escherichia coliand total Coliform bacteria, as indicated by the non-zero correlation value (R),although the influence was not significant due to a Sig. value > 0.05. Thedistribution pattern of bacteria was influenced by the direction of groundwaterflow and the direction of the river flow, which was towards the north, resulting inhigher contamination levels and an increasing trend towards the north.