B Background: Lemongrass leaves are a plant that is widely used as traditionalmedicine in Southeast Asia. In dentistry, lemongrass leaves are used as woundcare ingredients, with active ingredients such as essential oils, alkaloid acids,flavonoids, saponins, tanins, phenols, steroids, and terpenoids which play a role ininducing fibroblast cells to proliferate and use extracellular matrix for the healingprocess a wound. Objective: To determine the effect of lemongrass leaf extracton viability, cytotoxicity, proliferation, and migration of fibroblast cells.Methods: The study was divided into 5 groups namely lemongrass leaf extract200 μg/ml, 300 μg/ml, 400 μg/ml, 500 μg/ml, and without lemongrass leaf extract(control). Measurement of viability, cytotoxicity, and cell proliferation using aspectrophotometer, while cell migration was analyzed with T-scratch software.Results: The study showed a significant difference (p <0.05) between fibroblastcells given lemongrass extract compared with controls. Lemongrass leaf extractcan increase cell viability, reduce cytotoxicity, and increase fibroblast cellproliferation. In the migration test results, exposure to lemongrass leaf extractwith a concentration of 200 µg / ml, 300 µg / ml, 400 µg / ml, 500 µg / ml is ableto influence the acceleration of migration of fibroblast cells, the higher theconcentration the higher the migration ability of fibroblast cells. Conclusion: Thehigher the concentration of lemon grass leaf extract, the faster the growth offibroblast cells.