B Background: Injuries to the oral mucosa can be caused by various factors. Woundhealing is divided into three phases, namely inflammation, proliferation, andremodeling. Fibroblasts are cells found in tissues and are key in the wound healingprocess. Silver particles can be physically modified into silver nanoparticles andreduced through biological synthesis using natural ingredients containing antioxidantactive compounds such as lemongrass leaves (Cymbopogon citratus). Synthesis ofsilver nanoparticles from citronella leaf extract (NPsCc) is known to have goodcharacteristics and is able to increase the viability, proliferation, and migration offibroblasts in the wound healing process. Objectives: To determine the characteristicsand antioxidant activity of NPsCc and to determine the effect of synthesis of silvernanoparticles from lemongrass leaf extract on cytotoxicity, viability, proliferation,and migration of fibroblasts. Methods: The study was divided into 8 groups, namelythe untreated group (negative control), the control group that was given a stressor ofhydrogen peroxide, ascorbic acid (positive control), and five NPsCc treatment groupswith concentrations of 7.5 ppm, 15ppm, 30ppm, 60ppm, 120ppm. Particlecharacteristic test with Particle Size Analyze (PSA). Viability/cytotoxicity test,proliferation using CCK-8 at a wavelength of 517 nm, migration test performed usingwound healing method Results: NPsCc particles were nanoparticles withmonodisperse distribution and stable. The IC50 values of antioxidants andcytotoxicity of NPsCc were 231.91 g/mL and 44.87μg/mL, respectively. The resultsof the viability and proliferation and migration tests showed that NPsCc with aconcentration of 15 ppm was able to increase the viability, proliferation and migrationof fibroblasts Conclusion: NPsCc has very weak antioxidant but can increasefibroblas proliferation, viability, and migration of fibroblast at NPsCc concentrationof 15 ppm.