T Temulawak is one plant species that is common in the lives of south east asian people. Essential oils of temulawak rhizome is thought to have antimicrobial effects. Phytochemical test results show that temulawak rhizome contains alkaloids, saporin, phenolic, flovanoid, trierpenoid and glycosides which were able to inhibit the growth of bacteria. The purpose of this study is determine the effect of antibacterial essential oil of temulawak rhizome against bacteria Porphyromonas gingivalis. Rhizome of temulawak essential oil is obtained by distillation. The concentration of essential oil of temulawak rhizome is composed of 25%, 30%, 35%, 40%, 45% and 50%. At the bacterial activity test, performed using disc diffusion on blood agar medium inoculated with the bacterium Porphyromonas gingivalis. Positive and negative control used is chlorhexidine 0.2% and distilled water. The whole disc is incubated at 37° C for 72 hours. Inhibition zone formed was measured with calipers. Diameter zone of inhibition of bacteria at a concentration of 25%, 30%, 35%, 40%, 45% and 50% respectively as 7.15 mm, 7.70 mm, 8.58 mm, 9.99 mm, 10.02 mm, 10.69 mm. In chlorhexidine 0.2%, the diameter of inhibition zone formed is 15.15 mm. The data was analyzed using one way ANOVA test and Tukey HSD. Based on these results, the essential oil of temulawak rhizome has an antibacterial effect on the growth of the bacteria Porphyromonas gingivalis however, the antibacterial effect of essential oil of temulawak rhizome with a concentration of 50% has not been able to match the antibacterial effect of chlorhexidine 0.2%.