E nterobiasis is a parasitic infection caused by Enterobius vermicularis and is the most common cause of helminthiasis in children in developing countries. The prevalence of this worm is high throughout the world, especially in temperate and tropical regions. The prevalence of enterobiasis in Indonesia by 3% -80% covers various population groups, with the most age groups infected being between 5-9 years. One factor that is associated with enterobiasis in children is the level of education and knowledge of the mother. Enterobiasis can be examined using an anal swab examination. The purpose of this study was to determine the relationship between mother's level of education and knowledge with the incidence of enterobiasis in children. This research is an analytic study with cross sectional design on 155 mothers and students 1-4 grades in SD Bintang Timur. Enterobiasis examination uses an anal swab. Measurement of mother's education level uses an identity form. Measurement of the level of maternal knowledge using a questionnaire taken from Korean J Parasitology. Data analysis was performed univariately and bivariately with the Kolmogorov-Smirnov test using the SPSS program. Most respondents (53.5%) were 5-7 years old. Respondents with infection with hobbyasis were 3.2%, low education level was 20.6%, and the level of lack of knowledge was 5.8%. The Kolmogorov-Smirnov test showed that there was a significant relationship between the level of education of the mother and enterobiasis in children (p = 0.003), and a significant relationship between the level of knowledge of the mother and enterobiasis in children (p = 0,000). There is a significant relationship between the level of education, and mother's knowledge, with the incidence of enterobiasis in children at SD Bintang Timur.