I n dental care, dentists as we all may know, have main role in the dental health service as professionals. But, there is one of the alternative option of dental services known as traditional dental practitioners are widely spread in Indonesia and become public interest because it costs lower than the professional dentists. However, the government had made rules about traditional dental practitioners’s safety of work and their instruments. For example, they can clean their working instruments with correct way such as using autoclave, chemical steam, and other. Proper storage area is also one of supporting factors of the sterilization. In this study, we used observational method, to evaluate how the sterilization perfomed on traditional dental practitioners daily work. To find out the result, the research method is done by giving interviews and distribution of questionnaires to 20 traditional dental practitioners. The result showed that the level of sterilization performed by traditional dental practitioners in Palangka Raya is low. Most traditional dental practitioners use water to sterilize their working instruments carelessly on the table or in plastic. Just a few of them are actually care and understand the important of safety and hygiene of the working instruments because of the minimum education and knowledge that they have received.