B Breast cancer is one of the cancers that most frequently attacks women. In Indonesia is the second reason of death to women. It has become bigger problem because the patients going to the doctor are in the late stadium. This research applied Breast self-examination (BSE) is a screening method used in an attempt to detect early breast cancer. A motivated women who is concerned about her health and well-being will more likely be in favor of praticing of BSE in the future and on a regular basis. The purpose of this study was to assess the level of knowledge regarding breast cancer with interest in doing BSE among female students in the Darussalam Islamic High School, Bekasi.METHODThis type of research is analytical descriptive study with cross-sectional design with a sample of 79 female students in the Darussalam Islamic High School, Bekasi. Data were collected by questionnaires which include exposure to assessment of knowledge breast cancer, and interest in doing BSE.RESULTSKhi Kuadrat test and regression analysis test showed of 79 respondents who obtained results have good knowledge (52,8%), moderate and less of knowledge (47,17%). Their interest in doing BSE the category of good (65,2%) while the rest including the category of moderate and less (34,8%).CONCLUSIONWith this study it can be concluded that there is relationship between the level of knowledge about breast cancer and interest in doing BSE, 2count = 31,69 (2table < 9,488). There is relationship between knowledge about breast cancer and grade class in High School, 2count = 15,14. There is relationship between interest in doing BSE and grade class in High School, 2count = 10,20.