T There are three main sources of crude oil contamination in marine environment, which are natural processes; intentional discharge; and tanker casualties. These conditions create major loss in many aspects, especially on the ecosystem of marine environment. Po/ycyclyc Aromatic Hydrocarbon (PAH) phenanthrene is one of crude oil's component which is potentially carcinogenic and persistent in nature environment. Therefore, engineers should clean oil spills up for example with widely known bioremediation technique, such as: biostimulation and bioaugmentation. Indigenous bacteria have important role in a biostimulation process. Therefore, we need to characterize the potential indigenous isolates and study their physiologies attributes. Main steps of this research are isolation of indigenous Pari sea's ~acteria capable on degrading phenanthrene; ability reconfirm test and growth test; the identification and characterization of bacteria; Phenanthrene's biodegradation test with variation of KN03 or NH4N03 as nitrogen sources. Two isolates capable on degrading phenanthrene were identified as Ruegeria sp. strain 00898 and Alpha Proteobacterium strain GMDJE10F1. Alpha Proteobacterium GMDJE10F1 is a cosmopolit bacteria, which can optimally grows on temperature 25°C and 2% salinity with a biomass increase from 1,0P-7 /ml to 1,24E+9/ml and µ = 0,118/hour. Ruegetia sp. 00898 is a seawater bacteria, which can optimally grows on temperature 25°C and 2% salinity, with a biomass increase from 5,0E+6/ml to 1,43cr9/ml and µ = 0,2099/hour. After 19 days, biodegradation of phenenthrene with the variation on nitrogen sources (KN03 or NH4NC>.3) by each bacteria are in a range between 65,23%--70,70%. The highest efficiency of PAH phenanthrene's biodegradation (70,70%) were done by Ruegeria sp. 00898 with KN03 as nitrogen source, the degradation rate (v) is 1,15 mg/hour (k = 0,069/day), whereas the lowestefficiency of PAH phenanthrene's biodegradation (65,23%) were done by AlphaProteobacterium GMDJE10Fl with NH4N03 as nitrogen source, v = 0,99 mg/jam (k =0,059/hari). Although not significantly different, the efficiency of PAH Phenanthrene's biodegradation by the Ruegeria sp. 00898 is better than Alpha Proteobacterium GMDJElOFl. However, each of them can use both KN~ and NH4N03 in bioclegradation of phenanthrene. Also, both of them have a better result of biodegradation rate when using KN~ than NH4N03, as nitrogen sources. Hence, the formulation of fertilizer which contain KN~ compound will give an optimal process of PAH phenanthrene biodegradation by Pari Sea's indigenous bacteria.