M Mycobacterium tuberculosis (MTB) is a bacteria that infects the lungs causing respiratory problems in humans or is known as tuberculosis or TB. Primary care, such as community health centers, can carry out acid-fast bacilli (AFB) examinations and rapid molecular tests (TCM) for patients with suspected pulmonary TB. The samples used for examination use good quality sputum specimens. The research is expected to be able to determine the relationship between sputum quality and the results of AFB examinations using the ZN methods and TCM at East Bogor Public Health Center.METHODSThis research uses cross sectional. There were 53 samples with AFB examination results using the ZN methods and TCM in laboratory data. Data were analyzed using SPSS 27 with a chi-square significance test p<0.05.RESULTSThis study shows that the most common characteristics of sputum are clear color, mucopurulent/seropurulent consistency, and volume > 3 ml. There is a significant relationship between color (p=0.020), consistency (p=0.002), and volume (p=0.005) on AFB examination ZN method. In the TCM examination, there was a significant relationship between color (p=0.042), consistency (p=0.001), and volume (p=0.002) at the Puskesmas Bogor Timur.CONCLUSIONSThere is a relationship between sputum quality and the results of the BTA examination using the ZN and TCM methods. Further research is recommended with more thorough preparation.