G Groundwater is used by most people as a source of clean water and hygiene purposes. The quality of groundwater must be maintained, one indicator of the decline in groundwater quality is the presence of bacteria, including coliform bacteria and Escherichia coli. In 2019, Tanah Sareal District, one of the districts in Bogor City, had the highest diarrhea cases. This study aims to determine the quality of groundwater in Tanah Sareal District, Bogor City, determine the factors that influence bacterial content, analyze the relationship between well conditions (well depth, septic tank distance, pollutant source distance) with bacteria content and distribution patterns. Bacterial analysis using the APHA 9223 A method using IDEXX Colilert and MPN tables from IDEXX Quanty Tray 2000 to calculate the amount. Based on the results of research on groundwater quality in Tanah Sareal District, Bogor City does not meet the Quality Standards for the Minister of Health Regulation number 32 of 2017, the content of Escherichia coli and total Coliform ranges from 1 to >2500 MPN / 100 ml, these results exceed the standard standards the quality is 0 MPN / 100. The factors that cause the bacteria content are environmental sanitation conditions (distance of septic tanks and pollutant sources) and well construction conditions. The regression test results showed a relationship between well conditions and the content of Escherichia coli and total Coliform because the correlation value (R) ≠ 0., But the relationship was not significant because Sig. > 0.05. The distribution pattern of bacteria is influenced by the elevation and contour of the soil which affects the direction of groundwater flow.