Proporsi populasi  lactobasillus reuteri pada saliva subyek karies dan bebas karies (Laporan Penelittian)

DETAIL KOLEKSI

Proporsi populasi lactobasillus reuteri pada saliva subyek karies dan bebas karies (Laporan Penelittian)

5.0

Oleh : Phoebe Claudia

Info Katalog

Nomor Panggil : 616.904 1 CLA p

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2014

Pembimbing 1 : drg. Armelia Sari, M.Biomed.

Subyek : Microbiology

Kata Kunci : Clitoria ternatea, Streptococcus mutans, KHM, dan KBM Probiotik, lactobasilius reuteri, PCR, karis

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.	Link
1.	2014_TA_KG_04010154_Halaman-Judul.pdf
2.	2014_TA_KG_04010154_Bab-1.pdf
3.	2014_TA_KG_04010154_Bab-2.pdf
4.	2014_TA_KG_04010154_Bab-3.pdf
5.	2014_TA_KG_04010154_Bab-4.pdf
6.	2014_TA_KG_04010154_Bab-5.pdf
7.	2014_TA_KG_04010154_Bab-6.pdf
8.	2014_TA_KG_04010154_Bab-7.pdf
9.	2014_TA_KG_04010154_Daftar-Pustaka.pdf

Abstrak
Abstract

L Lactobacillus reuteri adalah salah satu bakteri probiotik yang mampu menekan pertumbuhan dari streptococcus mutans. Tujuan penelitian : untuk mengetahui perbedaan proporsi populasi L.reuteri pada saliva subyek karies dan bebas karies dan membuktikan bahwa L.reuteri merupakan flora normal rongga mulut.Pada penelitian ini dilakukan proses ekstraksi DNA dilanjutkan dengan amplifikasi gen 16s rRNA dari L.reuteri menggunakan metode PCR, lalu diamati dengan translluminator UV.

P Probiotic bacteria and pathogen bacteria is a normal oral flora. Probiotic have many benefits in improving oral health. Lactobacillus reuteri is one of probiotic bacteria that capable in supressing the growth of Streptococcus mutans. The purpose of this study is to determine the proportion difference of L. reuteri population between caries and caries free saliva subjects as well as to prove L. reuteri as the common oral microorganism. The subject in this study were 10 caries contracted subjects and 10 caries free subjects. DNA extraction were performed in this study and continued by 16s rRNA gene of L. reuteri using PCR methods, then the product were analyzed by UV translluminator. The result of caries free subjects showed two DNA L. reuteri band in 1100 bp. In the other hand, the result of caries subjects showed no band. The result showed the proportion difference of L. reuteri in human oral cavity between caries and caries free subjects as well as to prove L. reuteri as the common oral microorganism.