B Background: Cancer is ranked as the second leading cause of death worldwide,following cardiovascular diseases. An effective approach to cancerchemoprevention involves using natural compounds derived from plants. Amongsuch plants, Peperomia pellucida L. Kunth (P. pellucida), commonly referred to as“daun suruhan” has demonstrated significant potential as an anticancer agent.Phytochemical compounds in P. pellucida leaf extract has been reported to exhibitanticancer effect. Previous studies have shown the anticancer properties of P.pellucida againts several cell lines, including MCF-7. Objective: To assess thepotential effects of ethanol extract from P. pellucida leaves on cell viability andapoptosis in HSC-3 cell lines. Methods: HSC-3 cell line treated with ethanolextract of P. pellucida leaves at concentrations of 5, 10, and 20 µg/mL wereincubated for 24 hours. Subsequently, an MTT assay was conducted to evaluate cellviability, and Flow Cytometry analysis at the sub-G1 phase was performed todetermine the cells undergoing apoptosis. Results: The MTT assay results showedthe number of viable cells in the negative control (11,700), P. pellucida ethanolextract at 5 µg/mL (11,083), P. pellucida ethanol extract at 10 µg/mL (9,502), P.pellucida ethanol extract at 20 µg/mL (4,643), and positive control (987), with anIC50 value of 18.01 µg/mL. The Flow Cytometry results demonstrated apoptosispercentages in HSC-3 cell lines for the negative control (4.7%), P. pellucida ethanolextract at 5 µg/mL (13.0%), P. pellucida ethanol extract at 10 µg/mL (32.5%), P.pellucida ethanol extract at 20 µg/mL (61.2%), and positive control (82.5%). TheIC50 value measured in the Flow Cytometry assay was 16.33 µg/mL. Conclusion:The ethanol extract of P. pellucida showed significant efficacy in reducing cellviability and inducing apoptosis in HSC-3 cell lines. b