Efektivitas antibakteri ekstrak daun nicotiana tabacum terhadap aggregatibacter actinomycetemcomitans (penelitian in-vitro)

DETAIL KOLEKSI

Efektivitas antibakteri ekstrak daun nicotiana tabacum terhadap aggregatibacter actinomycetemcomitans (penelitian in-vitro)

Oleh : Theresa Christy Vinsensius

Info Katalog

Nomor Panggil : 617.634 2 THE e

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2025

Pembimbing 1 : Wiena Widyastuti

Subyek : Conservative dentistry

Kata Kunci : calcium hydroxide, Nicotiana tabacum leaf extract, Aggregatibacter actinomycetemcomitans, antibacter

Status Posting : Published

Status : Lengkap

File Repositori

No.	Nama File	Hal.
1.	2025_SK_SIG_040002100115_Halaman-Judul.pdf
2.	2025_SK_SIG_040002100115_Surat-Pernyataan-Revisi-Terakhir.pdf	1
3.	2025_SK_SIG_040002100115_Surat-Hasil-Similaritas.pdf	1
4.	2025_SK_SIG_040002100115_Halaman-Pernyataan-Persetujuan-Publikasi-Tugas-Akhir-untuk-Kepentingan-Akademis.pdf	1
5.	2025_SK_SIG_040002100115_Lembar-Pengesahan.pdf	1
6.	2025_SK_SIG_040002100115_Pernyataan-Orisinalitas.pdf	1
7.	2025_SK_SIG_040002100115_Formulir-Persetujuan-Publikasi-Karya-Ilmiah.pdf	1
8.	2025_SK_SIG_040002100115_Bab-1.pdf
9.	2025_SK_SIG_040002100115_Bab-2.pdf
10.	2025_SK_SIG_040002100115_Bab-3.pdf
11.	2025_SK_SIG_040002100115_Bab-4.pdf
12.	2025_SK_SIG_040002100115_Bab-5.pdf
13.	2025_SK_SIG_040002100115_Bab-6.pdf
14.	2025_SK_SIG_040002100115_Bab-7.pdf
15.	2025_SK_SIG_040002100115_Daftar-Pustaka.pdf
16.	2025_SK_SIG_040002100115_Lampiran.pdf

Abstrak
Abstract

L Latar Belakang: Karies gigi yang merupakan masalah kesehatan utama di Indonesia dapat disebabkan oleh Aggregatibacter actinomycetemcomitans, bersama dengan bakteri penyebab karies lainnya dapat menurunkan pH mulut dan menyebabkan kerusakan gigi. Kalsium hidroksida (Ca(OH)2) memiliki sifat antibakteri kuat dan merupakan standar baku bahan perawatan pulpa vital, namun memiliki efek samping yaitu nekrosis jaringan pulpa. Sehingga diperlukan alternatif pengobatan dari bahan alam seperti ekstrak daun tembakau (Nicotiana tabacum) yang memiliki kandungan flavonoid, saponin, steroid, dan terpenoid yang memberikan sifat antibakteri. Tujuan: Mengetahui adanya aktivitas antibakteri daun Nicotiana tabacum terhadap Aggregatibacter actinomycetemcomitans. Metode: Jenis penelitian ini merupakan penelitian laboratorium eksperimental in vitro dengan rancangan post-test-only control group design. Pengujian aktivitas antibakteri dilakukan dengan metode plate count menggunakan sampel dari tujuh kelompok yang berbeda. Sampel yang digunakan adalah ekstrak daun Nicotiana tabacum dengan konsentrasi 3,125%, 6,25%, 12,5%, 25%, dan 50%, kalsium hidroksida sebagai kontrol positif, dan akuades sebagai kontrol negatif. Hasil: Ekstrak daun Nicotiana tabacum memiliki aktivitas antibakteri terhadap Aggregatibacter actinomycetemcomitans. Penelitian membuktikan bahwa ekstrak daun Nicotiana tabacum dengan konsentrasi 12,5% merupakan Kadar Hambat Minimum (KHM) dan konsentrasi 25% merupakan Kadar Bunuh Minimum (KBM). Pada konsentrasi 25% dan 50% memiliki efek antibakteri terhadap Aggregatibacter actinomycetemcomitans yang setara dengan kalsium hidroksida. Kesimpulan: Terdapat efek antibakteri ekstrak daun Nicotiana tabacum terhadap Aggregatibacter actinomycetemcomitans.Kata kunci: kalsium hidroksida, ekstrak daun Nicotiana tabacum, Aggregatibacter actinomycetemcomitans, efek antibakteri.

B Background: Dental caries, which is a major health issue in Indonesia, can be caused by Aggregatibacter actinomycetemcomitans, along with other caries-causing bacteria that lower the mouth\\\'s pH and cause tooth damage. Calcium hydroxide (Ca(OH)2) has strong antibacterial properties and is the standard material for vital pulp treatment; however, it has side effects such as pulp tissue necrosis. Therefore, there is a need for alternative treatments using natural materials such as tobacco leaf extract (Nicotiana tabacum), which contains flavonoids, saponins, steroids, and terpenoids that provide antibacterial properties. Purpose: To determine the antibacterial activity of Nicotiana tabacum leaves against Aggregatibacter actinomycetemcomitans. Methods: This study is an in vitro experimental laboratory research with a post-test-only control group design. The antibacterial activity was tested using the plate count method on samples from seven different groups. The samples used included Nicotiana tabacum leaf extract at concentrations of 3,125%, 6,25%, 12,5%, 25%, and 50%, with calcium hydroxide as positive control and distilled water as negative control. Results: Nicotiana tabacum leaf extract exhibits antibacterial activity against Aggregatibacter actinomycetemcomitans. Studies have shown that a 12,5% concentration of the extract represents the Minimum Inhibitory Concentration (MIC), while a 25% concentration represents the Minimum Bactericidal Concentration (MBC). Concentrations of 25% and 50% exhibit antibacterial effects against Aggregatibacter actinomycetemcomitans comparable to calcium hydroxide. Conclusion: There is an antibacterial effect of Nicotiana tabacum leaf extract against Aggregatibacter actinomycetemcomitans.Keywords: calcium hydroxide, Nicotiana tabacum leaf extract, Aggregatibacter actinomycetemcomitans, antibacterial effects.