DETAIL KOLEKSI

Ekspresi protein membran biomarker CD90 pada sel punca pulpa (Laporan penelitian)


Oleh : Jasmine Shafira

Info Katalog

Nomor Panggil : 612.015 JAS e

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2018

Pembimbing 1 : Ferry Sandra

Subyek : Dental pulp;Stem cells

Kata Kunci : dental pulp stem cells, mesenchymal stem cells, flow cytmetry, biomarker

Status Posting : Published

Status : Lengkap


File Repositori
No. Nama File Hal. Link
1. 2018_TA_KG_040001400082_Halaman-Judul.pdf
2. 2018_TA_KG_040001400082_Lembar-Pengesahan.pdf
3. 2018_TA_KG_040001400082_Bab-1_Pendahuluan.pdf
4. 2018_TA_KG_040001400082_Bab-2_Tinjauan-pustaka.pdf
5. 2018_TA_KG_040001400082_Bab-3_Kerangka-teori,-konsep,-dan-hipotesis.pdf
6. 2018_TA_KG_040001400082_Bab-4_Metode-penelitian.pdf
7. 2018_TA_KG_040001400082_Bab-5_Hasil-penelitian.pdf
8. 2018_TA_KG_040001400082_Bab-6_Pembahasan.pdf
9. 2018_TA_KG_040001400082_Bab-7_Kesimpulan-dan-saran.pdf
10. 2018_TA_KG_040001400082_Daftar-Pustaka.pdf 3
11. 2018_TA_KG_040001400082_Lampiran.pdf

S Sel punca terbukti memiliki peran yang besar terhadap pertumbuhan dan perkembangan gigi. Penelitian ini bertujuan untuk mengetahui ekspresi protein membran biomarker CD90 pada SPP. Identifikasi sel dilakukan dengan teknik fluorescent antibody cell sorting dimana sel diidentifikasi dan diisolasi dari populasi sel yang beragam dan ditangkap menggunakan antibody spesifik terhadap CD90. Sampel dibagi menjadi tiga kelompok uji yaitu sampel unstained, antibody isolatype, dan cocktail. Setiap kelompok uji diperiksa sebanyak tiga kali. Analisis data dilakukan dengan pemeriksaan grafik scatter plots dan histogram yang dihasilkan dari BD stemflow Hmsc KIT. Hasil penelitian menunjukkan presentase ekspresi CD90 sebesar 97%, 97,2%, dan 97% untuk kelompok uji sampel coktail. Dari hasil penelitian dapat disimpulkan bahwa sampel yang didapat dari sel punca pulpa molar ketiga FKG Usaki telah memenuhi salah satu kriteria biomarker positif SPM yang diusulkan oleh ISCT yaitu ekspresi CD90.

S Stem cells have been shown to have a major role in the growth and development of teeth. This study aims to determine the expression of the CD90 biomarker membrane protein in SPP. Cell identification was carried out using a fluorescent antibody cell sorting technique in which cells were identified and isolated from diverse cell populations and captured using specific antibodies against CD90. The samples were divided into three test groups, namely unstained samples, isolatype antibodies, and cocktails. Each test group was examined three times. Data analysis was carried out by examining scatter plots and histograms generated from the BD stemflow Hmsc KIT. The results showed that the percentage of CD90 expression was 97%, 97.2%, and 97% for the cocktail sample test group. From the results of the study, it can be concluded that the samples obtained from the third molar pulp stem cells of FKG Usaki have met one of the criteria for a positive SPM biomarker proposed by ISCT, namely CD90 expression.

Bagaimana Anda menilai Koleksi ini ?