U Urinary tract infections in Indonesia are one of the most common types of nosocomial infections, with the number of cases varying between 90 and 100 cases per 100,000 people, which is equivalent to 180,000 cases every year. Antibiotic resistance is the most frequent cause of urinary tract infections in the world; therefore, it is important to know the bacterial profile of urinary tract infection patients and their sensitivity patterns to antibiotics.METHODSDescriptive observational research with a cross-sectional approach. The samples in this study consisted of 41 medical records of patients with urinary tract infections who had the results of urine culture in the period January 2020 to October 2023 at the RSAL Dr. Mintohardjo, Central Jakarta.RESULTSFrom 41 samples, 41 bacteria were found that cause urinary tract infections, including 35 gram-negative bacteria and 6 gram-positive bacteria. The most common gram-negative bacteria are Escherichia coli (53.7%), followed by other gram-negative bacteria such as Klebsiella spp. (14.6%), Enterobacter spp. (4.9%), Proteus mirabilis (4.9%), Serratia spp. (4.9%), and Citrobacter braakii (2.4%). Meanwhile, gram-positive bacteria that cause urinary tract infections include Staphylococcus spp. (14.6%). Gram-negative bacteria that cause urinary tract infections are most sensitive to the antibiotics Meropenem (94.3%), Amikacin (88.6%), Cefoxitin (82.9%), Piperacillin/Tazobactam (80,0%), and are resistant to Cefazolin (100%), Ampicillin (82.9%) and Ceftriaxone (74,3%). Meanwhile, gram- positive bacteria that cause UTIs are most sensitive to the antibiotic Vancomycin (100%) and resistant to Azithromycin (83.3%) and Penicillin (83.3%).CONCLUSIONSThe most common gram-negative bacteria that cause urinary tract infections is Escherichia coli, with antibiotics that have a sensitivity of >70%, including meropenem, amikacin, cefoxitin, and piperacillin/tazobactam. Meanwhile, gram- positive bacteria are Staphylococcus spp. with antibiotic sensitivity >70%, namely vancomycin.