Aktivitas antibiofilm reuterin isolat lactobacillus reuteri terhadap biofilm enterococcus faecalis dan fusobacterium nucleatum pada saluran akar gigi
Nomor Panggil : 616.904 1 ANA a
Penerbit : FKG - Usakti
Kota Terbit : Jakarta
Tahun Terbit : 2020
Pembimbing 1 : Dr. drg. Armelia Sari Widyarman, M.Kes.
Pembimbing 2 : C. Jayampath Seneviratne, BDS, M.Phil., Ph.D.
Subyek : Endodontics
Kata Kunci : enterococcus faecalis, fusobacterium nucleatum, irrigating solution, lactobacillus reuteri, probioti
Status : Lengkap
Status Posting : Published
No. | Nama File | Hal. | Link |
---|---|---|---|
1. | 2020_TA_KG_040001600083_Halaman-Judul.pdf | ||
2. | 2020_TA_KG_040001600083_Lembar-Pengesahan.pdf | ||
3. | 2020_TA_KG_040001600083_BAB-1_Pendahuluan.pdf | 5 | |
4. | 2020_TA_KG_040001600083_BAB-2_Tinjauan-Pustaka.pdf |
|
|
5. | 2020_TA_KG_040001600083_BAB-3_Kerangka-Teori,-Kerangka-Konsep,-Hipotesis.pdf |
|
|
6. | 2020_TA_KG_040001600083_BAB-4_Metode-Penelitian.pdf | 5 |
|
7. | 2020_TA_KG_040001600083_BAB-5_Hasil-Penelitian.pdf |
|
|
8. | 2020_TA_KG_040001600083_BAB-6_Pembahasan.pdf |
|
|
9. | 2020_TA_KG_040001600083_BAB-7_Kesimpulan-dan-Saran.pdf |
|
|
10. | 2020_TA_KG_040001600083_Daftar-Pustaka.pdf | 6 | |
11. | 2020_TA_KG_040001600083_Lampiran.pdf |
|
L Latar Belakang: Banyak larutan irigasi yang telah terbukti efektivitasnya untuk perawatan endodontik. Akan tetapi Enterococcus faecalis dan Fusobacterium nucleatum memiliki kemampuan untuk membentuk biofilm yang menjadipenyebab utama persistensi bakteri endodontic terhadap larutan irigasi. ProbiotikLactobacillus reuteri menunjukan kemampuan yang menjanjikan dalam mengatasipenyakit yang berhubungan dengan biofilm pada rongga mulut serta telah terbuktiampuh dalam mengatasi gingivitis dan mengurangi indeks plak. Tujuan: Penelitianini bertujuan untuk mengetahui aktivitas antibiofilm reuterin isolat Lactobacillusreuteri terhadap biofilm Enterococcus faecalis dan Fusobacterium nucleatum padasaluran akar gigi.Metode: Sebanyak 60 sampel gigi premolar dikumpulkan, dipreparasi, dandisterilisasi. Kemudian seluruh sampel gigi di inokulasi dengan monospecies sertamixed-species Enterococcus faecalis dan Fusobacterium dan dikultur selama24 jam. sampel gigi tersebut dibagi dalam 3 kelompok berdasarkan bahan irigasiyang diberikan, kelompok 1 diirigasi dengan NaOCl 2,5% (kontrol positif),kelompok 2 dengan saline (kontrol negatif), kelompok 3 dengan reuterin sebagaivariabel bebas. Sampel tersebut diambil dalam 5 menit dan 30 menit paska-irigasiuntuk dilakukan perhitungan koloni dalam CFU/mL. setelah itu total target DNAE. faecalis dan F. diekstraksi dan diidentifikasi menggunakan Real-time PCR.Hasil: Aktivitas antibiofilm reuterin isolat Lactobacillus reuteri terhadap biofilmEnterococcus faecalis dan Fusobacterium ditunjukan secara maksimal padakonsentrasi 100 µg/ml. Aktivitas antibiofilm yang ditunjukan oleh reuterin serupadengan NaOCl 2,5% sebagai kontrol positif. Disamping itu reuterin juga mampumengeradikasi perlekatan dan menghambat pertumbuhan biofilm E. faecalis danF. nucleatum (in vitro).Kesimpulan: Penelitian ini menunjukan bahwa reuterin isolat Lactobacillusreuteri memiliki potensi sebagai bahan irigasi dalam perawatan saluran akar gigi,melalui kemampuan antibiofilm yang dimilikinya.
B Backgrounds: Many irrigating solutions has proven its effectiveness forendodontic treatment, but Enterococcus faecalis and Fusobacteriumnucleatum have the ability to form biofilms that cause its persistence against someirrigating solution. Probiotic Lactobacillus reuteri has promising abilities tocombat many biofilm-related diseases in the oral cavity, as shown in many studiesthat have proven its abilities to reduce gingivitis and plaque index. Purpose: Theaim of this study is to investigate the antibiofilm activity of reuterinisolate Lactobacillus reuteri when used as an irrigating solutionagainst Enterococcus faecalis and Fusobacterium nucleatum biofilm in the rootcanal.Methods: A total of 60 human premolar teeth samples were collected, prepared,and sterilized. All teeth samples were inoculated with monospecies and mixedspecies of Enterococcus faecalis and Fusobacterium nucleatum and cultured for 24hours. Teeth were divided into 3 groups according to the irrigant type, group 1 was2.5% NaOCl (positive control), group 2 was saline (negative control), group 3 wasthe experimental reuterin isolate irrigant. The irrigation samples were obtained5 minutes and 30 minutes post-irrigation to determine the remaining colony formingunits of E. faecalis and F. nucleatum. Then a total DNA target of E. faecalis andF. nucleatum were extractes and identified using Real-time PCR.Results: Reuterin isolate Lactobacillus reuteri showed its antibiofilm activityagainst Enterococcus faecalis and Fusobacterium nucleatum biofilms with reuterin100 µg/ml as the optimal concentration. The antibiofilm activity demonstrated byreuterin resembled with NaOCl 2.5% as the positive control. Furthermore, reuterinalso showed its ability to eradicate and inhibit the growth of E. faecalis andF. nucleatum biofilm (in vitro).Conclusion: This study demonstrated that reuterin isolate Lactobacillusreuteri show potential in root canal therapy as root canal irrigant for its antibiofilmactivity.