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Induksi apoptosis sel kanker HeLa oleh secretome sel punca mesenkim darah tali pusat (Laporan penelitian)


Oleh : Elina Ardiani Sidharta

Info Katalog

Nomor Panggil : 616.07 SID i

Penerbit : FKG - Usakti

Kota Terbit : Jakarta

Tahun Terbit : 2014

Pembimbing 1 : Prof. drg. Janti Sudiono,, M.DSc.

Pembimbing 2 : drg. Ferry Sandra, Ph.D

Subyek : Pathology Anatomy

Kata Kunci : Mesenchymal stem cells, umbilical cord blood, secretome, HeLa cancer cells, apoptosis


File Repositori
No. Nama File Ukuran (KB) Status
1. 2014_TA_KG_04010067_lembar-pengesahan.pdf 666.88
2. 2014_TA_KG_04010067_daftar-isi-dll.pdf 732.06
3. 2014_TA_KG_04010067_bab-I.pdf 700.7
4. 2014_TA_KG_04010067_bab-II.pdf 1431.48
5. 2014_TA_KG_04010067_bab-III.pdf 660.19
6. 2014_TA_KG_04010067_bab-IV.pdf 833.46
7. 2014_TA_KG_04010067_bab-V.pdf 1500.92
8. 2014_TA_KG_04010067_bab-VI.pdf 755.95
9. 2014_TA_KG_04010067_bab-VII.pdf 627.43
10. 2014_TA_KG_04010067_daftar-pustaka.pdf 742.24
11. 2014_TA_KG_04010067_lampiran-1.pdf 1062.25
12. 2014_TA_KG_04010067_lampiran-2.pdf 1175.86
13. 2014_TA_KG_04010067_lampiran-3.pdf 631.57
14. 2014_TA_KG_04010067_lampiran-4.pdf 673.27

S Sel puca masekim (SPM) SPM diketahui menghasilakan produk yang dinamakan secretome yang dapat menginduksi apoptosis sel kanker. Namun banyak juga yang menyatakan bahwa SPM dapat mendukung pertumbuhan sel kanker dalam interaksinya . Penelitian ini dilakukan untuk mengevaluasi peran secretome dalam menginduksi apoptosis sel kanker. Pengetahuan mengenai mekanisme molekular dari secretome SPM dalam mempengaruhi microenvironment kanker masih kurang. Pada penelitian ini mengamati kemampuan SPM darah tali pusat (DTP) dalam menginduksi apoptosis sel kanker Hela. sel Hela di kultur dan diberi perlakuan dengan secretome SPM-DTP yang dikoleksi pada conditioned media (CM) dengan konsentrasi 0,2%,2%, 20% selama 24 atau 48 jam. Sel Hela yang mati akibat perlakuan tersebut dihitung dalam analis viabilitas sel dengan pewarnaan trypan blue.Transisi potensial tranmembran mitokondria (PTM) yang terjadi selama apoptosis dideteksi dalam analisis AѱM dengan pewarnaan DiOC₆ menggunakan flow cytometer. Hasil analisis viabilitas sel menunjukan bahwa CM SPM-DTP menginduksi kematian sel Hela, dan dijumpai peningkatan sel Hela yang yang mati seiring dengan peningkatan konsentrasi dan waktu perlakuan.

L Lately, mesenchymal stem cells (MSCs) are often associated with cancer along with increasing reports of their ability to be recruited not only to tissue injury, but also to the location of the cancer. MSCs are known to produce products called secretome that can induce apoptosis of cancer cells. However, there are also studies that report the effect of cancer cell growth in its interaction with MSCs. The molecular mechanisms of how MSCs secretome influences cancer cells’ microenvironment are still poorly known, that as to whether MSCs inhibit or induce apoptosis of cancer cells remains a controversy. Therefore, further research is needed to evaluate the role secretome in inducing apoptosis of cancer cells. This research observed the ability of human umbilical cord blood (hUCB) MSCs in inducing apoptosis of HeLa cancer cells. HeLa cells were cultured and treated with several concentrations of hUCB-MSCs secretome collected in conditioned media (CM): 0% (negative control), 0.2%, 2%, and 20% and a positive control H2O2 in two different time spans of 24 hours and 48 hours. Trypan blue staining was used to count and differentiate cells living and the dead. Sub-G1 analysis with Propidium Iodide staining was used to detect apoptopic cells. The data obtained showed hUCB-MSCs secretome induced apoptosis of cancer cells HeLa. hUCB-MSCs secretome induced apoptosis starting at 0.2% of hUCB-MSC secretome in 24 hour time (p<0.05). There was an interraction between the number of apoptotic HeLa cells with concentrations and long exposure time of secretome (p=0.000*<0.05). The higher the concentration of a given secretome and the longer the exposure time, more number of HeLa cells underwent apoptosis. In addition, it was found that the hUCB-MSCs secretome also caused partial proliferation inhibition of HeLa cells.

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